Description
TIRF creates an excitation energy field very close to the coverslip surface using total internal reflection from a laser beam. This energy is enough to excite fluorophores in the live cell that are in contact with the coverslip, but the rest of the sample remains unexcited (dark). This results in very low photoxicicity and very high resolution for imaging structures at the base of the cell such as focal adhesions, actin, microtubules and endocytic processes.
ManufacturerLEICA MICROSYSTEMS (UK) LTD
Model
