Centrifuge Ultracentrifuge Preparative

 

Description

Samples are centrifuged in cells which are transparent top and bottom. A laser scans the cells at regular intervals during centrifugation, recording changes in absorbance along the cell. A typical result from a sedimentation velocity experiment is shown at top right above. Early scans (in blue) show the boundary moving as molecules start to sediment; later scans (in red) show the molecules have been cleared from the top of the cell, giving a low absorbance, and accumulating at the bottom of the cell. Larger molecules (e.g. multimers) will tend to sediment faster than smaller ones; globular, folded molecules will sediment faster than asymmetric or unfolded molecules of the same molecular weight. Different rotor speeds are chosen for analysis of different sized molecules.

ManufacturerBECKMAN COULTER

ModelOPTIMA L-80XP

Contact

Academic Contact

Prof Prof Peter Stockley
p.g.stockley@leeds.ac.uk
+44-113-34-33092

Technical Contact

Dr Dr Iain Manfield
i.manfield@leeds.ac.uk
+44-113-34-32901